Fig 3. Innate immune cells in footpads of L. major infected WT and CatB^-/- mice.

WT and CatB^-/- were subcutaneously inoculated with 3x10⁶ stationary phase promastigotes of L. major in the footpads and experimental read-outs were measured weekly as indicated. (A) Gating strategy used for FACS analysis of footpad samples prepared from L. major -infected mice. (B) Percentage of CD45+ cells gated on live events from footpad single cell suspensions obtained from L. major -infected WT (filled bars) and CatB^-/- (empty bars) mice. (C-E) Percentage of Ly6G^-Ly6C^high monocytes (C), Ly6G^-Ly6C^int macrophages (D) and Ly6G^highLy6C^int neutrophils (E) gated on CD11b⁺CD11c^- cells from footpad single cell suspensions obtained from L. major -infected WT (filled bars) and CatB^-/- (empty bars) mice. Data depicted represent the mean and SEM of at least 2 independent experiments with n ≥ 4 mice/group/time point. * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001