Fig 5. Adaptive immune cell dynamics in responses to L. major infection of WT and CatB^-/- mice.

WT and CatB^-/- were subcutaneously inoculated with 3x10⁶ stationary phase promastigotes of L. major in the footpads and experimental read-outs were measured weekly as indicated. (A) Gating strategy used for analysis. Cell numbers in draining lymphnodes of L. major -infected WT (filled bars) and CatB^-/- (empty bars) mice (B). Percentages of CD19+ B cells (C), CD3+ T cells (D), CD4+ T cells (E) and CD8+ T cells (F) gated on the lymphocyte gate and foxp3+CD25+ Tregs (G) gated on the CD4+ gate from lymph nodes of L. major -infected WT (filled bars) and CatB^-/- (empty bars) mice. Data depicted represent the mean and SEM of at least 3 independent experiments with n ≥ 4 mice/group/time point. * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001