Effects of Ptf1a and Ptf1a-VP16 mRNA overexpression on organogenesis of liver, stomach and duodenum. (A-D) In situ hybridization for frp5 expression in control, Ptf1a-injected embryos and Ptf1a-VP16 injected embryos. (B) Normal expression is seen in the enlarged pancreas phenotype from Ptf1a-injected embryos, (C) whereas reduced expression is seen in the ectopic pancreas phenotype from Ptf1a-injected embryos. (D) No frp5 expression is detected in whole guts from Ptf1a-VP16 injected embryos. (E-H) In situ hybridization for Hex expression in Control, Ptf1a-injected embryos and Ptf1a-VP16 injected embryos. (F,G) Normal expression of Hex is detected in both enlarged and ectopic phenotypes from Ptf1a-injected embryos. (H) No Hex expression is detected in whole guts from Ptf1a-VP16 injected embryos.