Figure 7. p120 catenin family members localize to the membranes of epithelial cells in the absence of p120 catenin.

Immunofluorescent images demonstrating expression of p120 catenin family members in wild-type and homozygous p120^f/f pancreas. (A) ARVCF is expressed uniformly in the membranes of duct and islet cells and apically in some acinar cells in wild-type pancreas. It is also localized to the membranes of expanded duct cells and residual acinar cells in homozygous p120^f/f pancreas. (B) δ-catenin is expressed uniformly in the membranes of duct and islet cells and also in the apical and lateral membranes of some acinar cells in wild-type pancreas. In homozygous p120^f/f pancreas, δ-catenin localized to the membranes of expanded ducts. (C) p0071 was expressed uniformly in the membranes of duct and islet cells in wild-type pancreas. Expression of p0071 was also seen uniformly in the membranes of duct cells and remaining acinar cells in homozygous p120^f/f pancreas. Scale bars are 50μm. (D) Expression of ARVCF, δ-catenin, and p0071 in C^Pdx1; p120^f/f and wild-type pancreases was compared using qPCR. A significant reduction in expression of ARVCF, δ-catenin, and p0071 was observed in homozygous p120^f/f pancreases when compared to controls. Wild-type control targets were normalized to 1. For all genotypes and all genes, n=7, and reactions were run in quadruplicate. For interpretation of statistical results from Student’s t test, ns = not significant and p value > 0.05, * = p value ≤ 0.05, ** = p value ≤ 0.01, *** = p value ≤ 0.001, and **** = p value ≤ 0.0001.