. 2015 Dec 16;310(4):R355–R365. doi: 10.1152/ajpregu.00462.2015

Table 2.

Experiment 1: Morphometric and biochemical data for male DR rats injected at 4 wk of age with AAV control shRNA or AAV CTR shRNA in the VMH

	Sham	AAV Control	AAV CTR
6-wk chow
Initial body weight, g	87.6 ± 4.2	89.8 ± 3.8	84.4 ± 5.7
Body weight chow, g	306 ± 8	301 ± 12	287 ± 14
Body weight gain on chow, g	218 ± 9	212 ± 9	202 ± 11
Food intake on chow, kcal	2,629 ± 42	2,619 ± 110	2,505 ± 135
Feed efficiency on chow, BWG (g)/FI (kcal) × 1,000	83 ± 3	81 ± 3	81 ± 3
Leptin, ng/ml	5.14 ± 0.9^a	7.64 ± 0.89^a	15.8 ± 2.43^b
Glucose, mg/dl	137.63 ± 1.75	137.94 ± 7.46	138.69 ± 3.25
Insulin, ng/ml	0.80 ± 11^a	1.05 ± 0.10^a	1.85 ± 0.13^b
6-wk 45% fat diet
Body weight gain, g	126 ± 9^a	131 ± 18^a	168 ± 13^b
Food intake, kcal	1,594 ± 39	1,560 ± 72	1,613 ± 87
Feed efficiency, BWG (g)/FI (kcal) × 1,000	33 ± 1	35 ± 3	39 ± 5
Total 6-wk chow, 6-wk 45% fat diet
Final body weight, g	435 ± 14	433 ± 25	459 ± 23
Total body weight gain, g	347 ± 18	343 ± 23	375 ± 23
Total food intake, kcal	6,488 ± 173	6,310 ± 312	6,609 ± 370
Feed efficiency total, BWG (g)/FI (kcal) × 1,000	53 ± 1	54 ± 2	54 ± 3
Retroperitoneal, g	9.28 ± 0.78^a	10.52 ± 0.79^a	13.87 ± 1.22^b
Retroperitoneal, %BW	2.15 ± 0.17^a	2.43 ± 0.12^a,b	3.00 ± 0.22^b
Mesenteric, g	7.79 ± 1.45^a	8.39 ± 0.81^a	15.0 ± 1.7^b
Mesenteric, %BW	1.79 ± 0.16^a	1.94 ± 0.17^a	3.31 ± 0.31^b
Inguinal, g	6.47 ± 0.68^a	8.77 ± 1.01^a	16.89 ± 1.03^b
Inguinal, %BW	1.81 ± 0.34^a	2.05 ± 0.25^a	3.35 ± 0.28^b
Epididymal, g	9.38 ± 0.66	11.0 ± 1.3	16.0 ± 1.7
Epididymal, %BW	2.18 ± 0.15^a	2.53 ± 0.20^a	3.75 ± 0.34^b
Perirenal, g	3.50 ± 0.12	4.05 ± 0.52	5.70 ± 0.68
Perirenal, %BW	0.81 ± 0.04	0.93 ± 0.08	1.14 ± 0.09
Total fat pads, g	38.0 ± 3.3^a	42.8 ± 3.8^a	70.4 ± 8.5^b
Total fat pad/carcass weight, %	8.07 ± 0.45^a	9.88 ± 0.68^a	15.8 ± 0.9^b
Leptin, ng/ml	11.2 ± 0.7^a	18.8 ± 2.3^a	39.6 ± 2.9^b
Glucose, mg/dl	148 ± 4	152 ± 6	143 ± 3
Insulin, ng/ml	1.75 ± 0.28^a	2.63 ± 0.46^a,b	3.66 ± 0.46^b
Glucose AUC, mg·dl⁻¹·120 min⁻¹	4,932 ± 813	4,643 ± 606	5,731 ± 764
Insulin AUC, mg·dl⁻¹·120 min⁻¹	87.9 ± 19.7^a	91.1 ± 17.7^a	204.1 ± 30.6^b
Insulin sensitivity index	34.2 ± 5.2^a	30.9 ± 4.2^a	17.4 ± 2.5^b
Liver, g	13.64 ± 1.08	12.80 ± 0.89	12.66 ± 0.68
Naso-anal length, cm	24.8 ± 0.2	24.4 ± 0.4	23.6 ± 0.3

Values are means ± SE; n = 7 AAV control shRNA rats; n = 8 AAV CTR shRNA rats. Additional diet-resistant (DR) rats were sham-injected (n = 7). Rats were maintained on chow diet for 6 wk, followed by 6 wk on 45% fat diet.

VMH, ventromedial hypothalamus; AAV CTR shRNA, adeno-associated virus containing a short hairpin portion of the CTR mRNA; AAV, adeno-associated virus; BW, body weight; BWG, BW gain; FI, food intake; AUC, area under the curve.

^{^a,b}

Data for each parameter with differing superscripted letters differ from each other by P = 0.05 by post hoc Bonferroni test after intergroup differences were found by one-way ANOVA.