Table 3.
Experiment 1: Effects of VMH CTR depletion from 4 wk of age on arcuate gene expression in DR rats after 6 wk on chow and 6 wk on 45% fat diet
| Sham | AAV Control | AAV CTR shRNA | |
|---|---|---|---|
| CTRA | 2.92 ± 0.60a | 3.23 ± 0.70a | 0.37 ± 0.10b |
| CTRB | 2.16 ± 0.34a | 1.12 ± 0.21b | 0.12 ± 0.04c |
| RAMP1 | 1.20 ± 0.17a | 1.17 ± 0.15a | 0.90 ± 0.05b |
| RAMP2 | 1.28 ± 0.14a | 1.12 ± 0.07b | 1.31 ± 0.13a |
| RAMP3 | 0.94 ± 0.10 | 1.30 ± 0.20 | 1.11 ± 0.15 |
| NPY | 1.38 ± 0.32 | 1.02 ± 0.27 | 1.39 ± 0.25 |
| POMC | 1.75 ± 0.47 | 1.28 ± 0.48 | 1.03 ± 0.23 |
| AgRP | 1.51 ± 0.43 | 0.30 ± 0.45 | 1.24 ± 0.32 |
| IL-6 | 0.76 ± 0.09a | 2.82 ± 0.84b | 0.92 ± 0.19a |
| IL-6Rα | 0.72 ± 0.09 | 0.75 ± 0.12 | 1.01 ± 0.12 |
| gp130 | 0.93 ± 0.12 | 1.00 ± 0.21 | 0.95 ± 0.11 |
| Lepr-b | 1.09 ± 0.22 | 1.07 ± 0.20 | 1.22 ± 0.17 |
| SOCS3 | 0.86 ± 0.16 | 1.31 ± 0.34 | 1.03 ± 0.18 |
Values are means ± SE of duplicate determinations expressed relative to the amount of the mRNA expression of cyclophilin (n = 7–8/group).
a,b,c
Data for each parameter with differing superscripted letters differ from each other by P = 0.05 by post hoc Bonferroni test after intergroup differences were found by one-way ANOVA.