Table 4.
Experiment 1: Effects of VMH CTR depletion from 4 wk of age on ventromedial nucleus gene expression in DR rats after 6 wk on chow and 6 wk on 45% fat diet
| Sham | AAV Control | AAV CTR shRNA | |
|---|---|---|---|
| CTRA | 1.18 ± 0.25a | 1.73 ± 0.37a | 0.25 ± 0.13b |
| CTRB | 0.99 ± 0.19a | 1.30 ± 0.31a | 0.07 ± 0.04b |
| RAMP1 | 1.57 ± 0.29 | 1.75 ± 0.49 | 1.27 ± 0.23 |
| RAMP2 | 1.22 ± 0.10 | 0.90 ± 0.14 | 0.97 ± 0.12 |
| RAMP3 | 1.65 ± 0.17 | 1.55 ± 0.16 | 1.45 ± 0.16 |
| IL-6 | 0.77 ± 0.11 | 0.87 ± 0.13 | 0.82 ± 0.09 |
| IL-6Rα | 0.50 ± 0.16 | 1.06 ± 0.31 | 1.30 ± 0.30 |
| gp130 | 2.01 ± 0.19 | 2.38 ± 0.24 | 2.43 ± 0.23 |
| Lepr-b | 1.07 ± 0.13 | 0.95 ± 0.18 | 0.81 ± 0.12 |
| SOCS3 | 0.42 ± 0.11 | 0.74 ± 0.13 | 0.52 ± 0.03 |
Values are means ± SE of duplicate determinations expressed relative to the amount of the mRNA expression of cyclophilin (n = 7–8/group).
a,b
Data for each parameter with differing superscripted letters differ from each other by P = 0.05 by post hoc Bonferroni test after intergroup differences were found by one-way ANOVA.