. 2015 Dec 16;310(4):R355–R365. doi: 10.1152/ajpregu.00462.2015

Table 5.

Experiment 2: Morphometric and biochemical data for male sham-operated DIO and DR rats injected at 4 wk of age with control AAV shRNA or AAV CTR shRNA in the VMH

	DIO Sham	DR Ctrl AAV	DR AAV CTR shRNA
Initial body weight, g	86 ± 4^a	66 ± 2^b	65 ± 2^b
Final body weight, g	433 ± 11	288 ± 6^b	283 ± 8^b
Body weight gain, g	347 ± 9^a	222 ± 6^b	218 ± 8^b
Food intake, kcal	4,669 ± 115^a	3,221 ± 81^b	3,264 ± 112^b
Feed efficiency, BWG (g)/FI (kcal) × 1,000	74.3 ± 7.2	68.9 ± 6.9	66.8 ± 7.1
Perirenal, g	1.31 ± 0.14^a	0.45 ± 0.08^b	0.43 ± 0.04^b
Mesenteric, g	5.86 ± 0.30^a	2.81 ± 0.13^b	2.85 ± 0.16^b
Inguinal, g	5.72 ± 0.27^a	2.85 ± 0.16^b	3.08 ± 0.17^b
Epididymal, g	5.92 ± 0.32^a	2.62 ± 0.10^b	2.60 ± 0.21^b
Perirenal, g	1.31 ± 0.14^a	0.45 ± 0.08^b	0.43 ± 0.04^b
Retroperitoneal, g	4.64 ± 0.24^a	1.55 ± 0.13^b	1.87 ± 0.12^c
Retroperitoneal carcass, %	1.10 ± 0.11^a	0.52 ± 0.04^b	0.65 ± 0.05^c
Total fat pad total, g	25.8 ± 2.2^a	10.1 ± 0.4^b	11.6 ± 0.4^c
Total fat pad/carcass weight, %	5.9 ± 0.3^a	3.4 ± 0.1^b	4.0 ± 0.1^c
Leptin, ng/ml	16.2 ± 2.2^a	6.1 ± 0.7^b	14.9 ± 2.1^a
Glucose, mg/dl	154 ± 8^a	132 ± 5^b	130 ± 6^b
Insulin, ng/ml	1.45 ± 0.14^a	0.87 ± 0.11^b	1.12 ± 0.10^c
Glucose AUC, mg·dl⁻¹·120 min⁻¹	3,789 ± 540^a	2,398 ± 425^b	2,934 ± 469^b
Insulin AUC, mg·dl⁻¹·120 min⁻¹	110 ± 10^a	72 ± 5^b	90 ± 6^c
Insulin sensitivity index	19.4 ± 1.3^a	33.3 ± 3.5^b	25.5 ± 2.3^c
CTR mRNA	0.71 ± 0.08^a	1.40 ± 0.20^b	0.27 ± 0.11^c

Values are means ± SE; n = 12/group.

DIO, diet-induced obese. Rats were maintained on chow diet for 6 wk.

^{^a,b,c}

Data for each parameter with differing superscripted letters differ from each other by P = 0.05 by post hoc Bonferroni test after intergroup differences were found by one-way ANOVA. CTR mRNA levels are given as relative abundance normalized against cyclophilin.