Fig. 10.

ER stress enhances lipid droplet accumulation in cultured human renal angiomyolipoma cells. A: fluorescence imaging of lipid droplet accumulation in TRI102 and TRI103 cells. TRI102 and TRI103 cells were treated with culture media supplemented with 0, 50, 100, or 400 μM Na-oleate for 24 h. Lipid droplet accumulation was evaluated by staining cells with LipidTOX (green), which labels neutral lipid droplets. Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI; blue). Lipid droplet accumulation was greater in TRI102 cells. Displayed images are from the same experiment. Image acquisition settings were constant for each experiment. Results are representative of four separate experiments. B: cells were treated with culture media supplemented with 0, 50, 100, or 400 μM Na-oleate including either the proteasome inhibitor MG-132 (500 nM) or DMSO for 4 h. Lipid droplet accumulation was visibly enhanced by MG-132. Displayed images are from the same experiment. Image acquisition settings were constant for each experiment. Results are representative of two separate experiments.