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Functional evidence of pericyte origin of renal angiomyolipomas. A: Western blot of phosphorylated (Ser473 and Thr308) and nonphosphorylated Akt in TRI102 and TRI103 cells after 1 h of treatment with PDGF-BB (5 or 50 ng/ml) or RAD-001 (20 nM). Results are representative of three separate experiments. B: densitometric analysis of immunoblots in A. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < .0001 compared with control.
