Table 1.
AITC evokes bradyarrythymia via TRPA1-mediated parasympathetic reflex in conscious rats
| RR Interval, ms | AV Block, count | PR Interval, ms | |
|---|---|---|---|
| Control | |||
| Ambient | 145 ± 3 | 0 ± 0 | 44.4 ± 0.7 |
| Vehicle | 153 ± 3 | 0 ± 0 | 44.2 ± 0.8 |
| AITC | 270 ± 15* | 112 ± 37* | 93.1 ± 13* |
| n | 11 | 11 | 8 |
| Ruthenium red (4 mg/kg ip) | |||
| Ambient | 156 ± 9 | 0 ± 0 | 48.6 ± 1.9† |
| Vehicle | 159 ± 9 | 0 ± 0 | 48.6 ± 1.8† |
| AITC | 224 ± 9*† | 19 ± 9*† | 56.2 ± 2.2*† |
| n | 9 | 9 | 5 |
| Atropine (1 mg/kg ip) | |||
| Ambient | 149 ± 6 | 0 ± 0 | 46.8 ± 0.6 |
| Vehicle | 140 ± 5† | 0 ± 0 | 44.8 ± 0.5 |
| AITC | 156 ± 3*† | 0 ± 0† | 46 ± 0.7† |
| n | 5 | 5 | 4 |
| Ketamine (100 mg/kg ip) | |||
| Ambient | 304 ± 33† | N.D. | N.D. |
| Vehicle | 298 ± 35† | N.D. | N.D. |
| AITC | 310 ± 29 | N.D. | N.D. |
| n | 4 | ||
| Urethane (1.2 g/kg ip) | |||
| Ambient | 228 ± 5† | N.D. | N.D. |
| Vehicle | 228 ± 8† | N.D. | N.D. |
| AITC | 229 ± 10 | N.D. | N.D. |
| n | 5 | ||
| Decerebration | |||
| Ambient | 164 ± 10† | 0 ± 0 | 50.8 ± 2.5† |
| Vehicle | 170 ± 10† | 0.3 ± 0.3 | 50.5 ± 2.3† |
| AITC | 235 ± 26* | 10 ± 10*† | 54 ± 2.7† |
| n | 5 | 4 | 5 |
Values are mean ± SE. RR interval, atrioventricular (AV) block, and PR interval upon exposure to ambient, vehicle, and AITC (30 mM).
AITC, allyl isothiocyanate; transient receptor potential ankyrin 1 (TRPA1); N.D., data not determined.
*
P < 0.05, significant difference between AITC and vehicle responses.
†
P < 0.05, significant difference between control and pretreatments.