Table 1. TGA Results Converted into Nominal Grafting Densities ρgraft for Different Sizes and Purification Methods.
raw |
column
purified (FR2) |
dialyzed
(48 h) |
membrane
centrifugation (11×) |
precipitation/magnetic
decantation (7×) |
||||||
---|---|---|---|---|---|---|---|---|---|---|
TOC [% w/w] | ρgraft [NDA-PEG(5 kDa)/nm2] | TOC [% w/w] | ρgraft [NDA-PEG(5 kDa)/nm2] | TOC [% w/w] | ρgraft [NDA-PEG(5 kDa)/nm2] | TOC [% w/w] | ρgraft [NDA-PEG(5 kDa)/nm2] | TOC [% w/w] | ρgraft [NDA-PEG(5 kDa)/nm2] | |
9.6 nm | 93.8 | 14.2a | 65.9 | 1.8a | 37.4 | 0.6 | 35.9 | 0.5 | 46.1 | 0.8 |
7.1 nm | 94.5 | 11.9a | 91.1 | 7.1a | 40.0 | 0.5 | 39.1 | 0.5 | 50.1 | 0.7 |
4.9 nm | 93.3 | 6.8a | 89.5 | 4.1a | 44.8 | 0.4 | 38.9 | 0.7 | 59.8 | 0.7 |
3.5 nm | 95.7 | 7.7a | 87.9 | 2.5a | 54.1 | 0.4 | 59.7 | 0.5 | 76.1 | 1.1 |
Samples contain amounts of free NDA-PEG(5 kDa).