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. Author manuscript; available in PMC: 2016 May 16.
Published in final edited form as: J Pineal Res. 2014 Jan 18;56(3):246–253. doi: 10.1111/jpi.12117

Figure 7. Expression of B-Raf in MCF-7 and MDA-MB-231 human breast cancer cells.

Figure 7

Whole cell lysates were extracted from MCF-7 and MDA-MB-231 breast cancer cells incubated in RPMI-1640 medium supplemented with 10% FBS. Fifty micrograms of total cellular protein from MDA-MB-231 cells was electrophoretically separated on 10% SDS polyacrylamide gel, and transferred onto nitrocellulose membrane. Expression of B-Raf was detected by Western blot analyses using anti-B-Raf antibody at a 1:1000 dilution. The same blots were stripped and reprobed with anti-actin antibody. This figure is a Western blot analysis of B-Raf expression in MCF-7 and MDA-MB-231 breast cancer cells, and it is representative of 3 independent experiments.