Fig. S3.

Binding kinetics between various anti-CD47 reagents and immobilized mouse or human CD47 determined by surface plasmon resonance. Representative sensograms of serial dilutions of anti-CD47 nanobody clones binding to immobilized mouse CD47 (Top), anti-mouse CD47 antibody (miap301) binding to immobilized mouse CD47 (Bottom Left), and CV1 binding to immobilized mouse or human CD47 (Bottom Middle and Right, respectively). The sensograms were fit to a 1:1 binding model using the BIAevaluation software for derivation of binding kinetics reported in Table 1 and Table S1.