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. 2016 Apr 18;113(19):E2646–E2654. doi: 10.1073/pnas.1604268113

Fig. S6.

Fig. S6.

Anti-mouse CD47 antagonist antibody (miap301) does not potentiate macrophage-mediated ADCP of IFN-γ–treated B16F10 cells in vitro. Antibody-dependent phagocytosis of B16IFN-γ cells by bone marrow-derived BALB/c mouse macrophages treated with various combinations of tumor antigen-specific (αTRP-1) antibody and anti–PD-L1 blocking antibody (10F.9G4) with or without CD47 antagonist nanobody (A4) or antibody (miap301). Phagocytosis is quantified as the percentage of F4/80-positive macrophages that have engulfed CFSE-positive B16IFN-γ cells. The data shown are the mean (n = 3), and error bars indicate SD. ns, not significant (P > 0.05); ***P < 0.001; ****P < 0.0001 determined by one-way analysis of variance with Holm–Sidak’s multiple comparisons test in Prism comparing each group to the control PBS group.