Fig. S7.

Anti-mouse CD47 nanobody A4 binds a range of mouse hematopoietic cells but does not cause substantial red blood cell or platelet depletion. (A) Histograms depicting Alexa-647–labeled A4 (Top) and anti-mouse CD47 antibody (Bottom) or isotype control antibody (solid gray histograms) reactivity with various blood cell populations as determined by flow cytometry. (B and C) Quantification of hematocrit (B) and platelet (C) levels in mice treated with control nanobody (Ctr) or anti-CD47 nanobody A4. Mice (n = 3 per group) were injected i.p. with 200 μg of protein per day for consecutive 4 d, and blood was collected for quantification. Data represent mean ± SD, **P < 0.01 as determined using a two-tailed, unpaired t test assuming equal SD in Prism.