Table S1.

Binding affinity and kinetics between anti-mouse CD47 nanobody clones and immobilized mouse CD47

	Mouse CD47
Molecule	ka, M-1⋅s−1	kd, s−1	KD, M
A4 Nb	2.0 × 107	2.3 × 10−4	1.2 × 10−11
H5 Nb	1.4 × 107	7.3 × 10−4	5.3 × 10−11
H9 Nb	8.0 × 106	9.6 × 10−4	1.2 × 10−10
F2 Nb	—	—	Weak binding

Sensograms were fit to a 1:1 binding model for derivation of binding kinetics. Dash (—) indicates no significant or quantifiable binding.