Table 1.

Binding affinity and kinetics between anti-mouse CD47 nanobody A4, CV1, and anti-mouse CD47 antibody miap301 immobilized mouse or human CD47

	Mouse CD47			Human CD47
Molecule	ka, M-1⋅s−1	kd, s−1	KD, M	ka, M-1⋅s−1	kd, s−1	KD, M
A4 Nb	2.0 × 107	2.3 × 10−4	1.2 × 10−11	—	—	Weak binding
CV1	1.8 × 106	1.1 × 10−2	6.2 × 10−9	7.0 × 106	3.7 × 10−5	5.4 × 10−12
miap301	1.6 × 105	6.2 × 10−4	4.0 × 10−9	n.d.	n.d.	n.d.

n.d., not determined. Dash (—) indicates no significant or quantifiable binding. Sensograms were fit to a 1:1 binding model for derivation of binding kinetics.