Fig. S4.

HLTF silencing does not affect HIV-1 replication in macrophages. MDMs were treated with siRNA Ctrl or siRNA against HLTF for 24 h. The cells were then not infected (NI) or infected with either Yu-2 wt virus (WT) or Yu-2 ∆Vpr virus (∆Vpr). A second siRNA treatment was done 48 h after infection. Cell supernatants were collected at the indicated time points, and p24 levels were measured by ELISA. Error bars represent SEM of triplicates from one donor. Same results were obtained with a second donor.