Fig. S7.

eIF4E-binding surface for importin 8. (A) Comparison of the surface charge distribution on the dorsal surface (Top) and cap-binding pocket (Bottom) of apo- and cap-bound forms of eIF4E (PDB ID codes 2GPQ and 3AM7, respectively). Key residues used for recognition with ligands and partners are also shown. Acidic (negatively charged) and basic (positively charged) regions are in red and blue, respectively. (B–D) We examined the ability of mutants with known functions (cap-binding, dorsal surface recognition, etc.) to bind importin 8. Each mutant (displayed in B) was subjected to GST pull-down assay (C). (D) Inputs for GST and GST-tagged eIF4E (WT and mutants) are shown. Ct, C terminus; Nt, N terminus.