Fig. 4.
Electropore diffusion. (A) A single frame from an oSCR stack (61.7 Hz) of a diffusing electropore, recorded at 200 mV, overlaid with the tracking trajectory (blue, 0 s, to red, 16.2 s). The pink circle indicates the detected location of the spot in this frame. (Scale bar: 2 µm.) (B) Representative mean-squared displacement versus time plots. Open circles: 180 mV; filled squares: 200 mV; open lozenges: 260 mV; filled triangles: 330 mV. Mean Dlat = 0.67 µm2⋅s−1 (n = 46). We observe no obvious correlation of diffusivity with the applied potential. (C) Electroporation in a DPhPC/DPPG/cholesterol (1:1:1) phase-separated DIB. Median image of 500 frames recorded at 99.4 Hz. Electropores form in the (bright) Ld phase, diffusing around the (dark) Lo regions. Colored overlays show the trajectories of different tracked electropores. (Scale bar: 5 µm.)