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Correlating fluorescence and electrical recording. A triplet of pores at 200 mV generates an electrical signal that comprises the three individual pore currents, EA+B+C (red). These can be revealed using fluorescence data (FA, FB, and FC, black). Fluorescence traces are overlaid atop duplicates of the total electrical signal to ease visualization of its components. The image is the maximum intensity over 200 frames recorded at 32.9 Hz. (Scale bar: 25 μm.)
