Fig. S6.

Additional examples of homopolymer sequence reads. The experiment was carried out as described for Fig. 6 in the main text, using the same tagged nucleotides and polymerase–nanopore complexes. In this case, however, 3 mM SrCl₂ and 0.1 mM MnCl₂ were present on the trans side and 0.1 mM MnCl₂ was present on the cis side of the membrane. Deflections less than 10 ms were discounted. Letters shown in gray (the fourth “T” in the middle trace and the fourth “A” in the lower trace) in the extended primer strand indicate bases that were missed in these reads.