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Conformation of His64 in apo-hCA II: (A) 0 s, (B) 50 s, (C) 3 min, and (D) 10 min. The 2Fo-Fc (blue) and Fo-Fc (green) electron density maps are contoured at 1.0σ and 5.0σ, respectively. The 2Fo-Fc maps for waters are highlighted in pink for clarity. There is no evidence of a preferable His64 position (in vs. out) or W2′ (except maybe in D).
