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. 2016 Apr 25;113(19):5447–5452. doi: 10.1073/pnas.1522458113

Fig. 2.

Fig. 2.

Point mutations in Prp8 are responsible for suppressors of atprmt5 mutants. (A) Sequence alignment of the conserved Prp8 containing the P347S and P1141S mutations in prp8-9 and prp8-8, respectively. Abbreviations for species are as follows: Arabidopsis thaliana (At), Oryza sativa (Os), Homo sapiens (Hs), Mus musculus (Mm), Drosophila melanogaster (Dm), Caenorhabditis elegans (Ce), Schizosaccharomyces pombe (Sp), Saccharomyces cerevisiae (Sc), and Plasmodium falciparum (Pf). The point mutation sites are labeled by black triangles. (B and C) Rescued growth retardation of young seedling leaves at 12 d (B) and intron retention events (C) by a transformation of CDS Prp8 clone with P1141S mutation in atprmt5-2 sus2-5 double mutants background. C8 and C20 are two individual transgenic lines containing P1141S construct of Prp8. W5 is the control transgenic line containing WT Prp8 construct.