Fig. S1.

Phenotypic and molecular analysis of atprmt5 suppressors. (A) Flowering time of Col, atprmt5 mutants, s215, and m90, assessed by total leaf number after plants stop producing new leaves. Plants were subjected to vernalization treatment at 4 °C for 6 wk and then transferred to 23 °C and grown under long day conditions. Data are shown as means ± SD (n = 20). Two-sided Student t test between atprmt5 and the suppressors was performed (**P < 0.01). (B) Comparison of the survival rate of the Col, atprmt5 mutants, s215, and m90 plants grown on MS medium containing 120 mM NaCl. Photographs were taken 25 d after seedling transfer to the treatment medium. (C) RT-PCR analysis for Prp8 RNAi lines in atprmt5 background and atprmt5-2 prp8-6 double mutants (in Ler background). (D) Rescued primary roots of the transformation lines of CDS Prp8 clone with P1141S mutation in atprmt5-2 sus2-5 double mutants background at 9 d. (E–G) Phenotypic analysis of the young seedling leaves at 12 d (E), the primary roots at 9 d (F), and the survival rate on MS medium containing 120 mM NaCl (G) for prp8-8 and prp8-9 mutants.