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. 2016 Apr 25;113(19):5447–5452. doi: 10.1073/pnas.1522458113

Fig. S2.

Fig. S2.

Low-resolution mapping and resequencing of the m90 mutation indicate it has a lesion in At1g80070. (A) (Upper) RNA blot analysis of the splicing patterns of At2g17340 in the F2 mapping population of m90, with rRNA as the loading control. The lanes marked with asterisks represent the population used for low-resolution mapping. The F2 population segregated into WT and mutant phenotypes at a ratio of 3:1, indicating that the mutant phenotype is caused by a single recessive mutation; (Lower) Bulked segregant analysis maps atprmt5-2 prp8-8 to between 1-AC002986-9779 and 1-AC011713-9987 on chromosome 1. (B) Phylogenic analysis of Prp8 from yeast, fly, mouse, worm, human, Arabidopsis, and rice. The species for each protein is designated by the following prefixes: Arabidopsis thaliana (At), Oryza sativa (Os), Homo sapiens (Hs), Mus musculus (Mm), Drosophila melanogaster (Dm), Caenorhabditis elegans (Ce), Schizosaccharomyces pombe (Sp), Saccharomyces cerevisiae (Sc), and Plasmodium falciparum (Pf).