Fig. S6.

Preparation of anti-SDMA antibody, U snRNAs analysis, and working model. (A) Low complex GAR motifs harboring symmetric dimethylarginines (SDMA). (B) Total proteins extracted from Col and atprmt5 mutants were immunoblotted with anti-SDMA and anti-SmD1 antibodies. (C) Levels of U1, U2, U4, U5, and U6 snRNAs are increased in atprmt5 mutants and restored in atprmt5 prp8-8. 18S rRNAs were used as a loading control. (D) Model for the role of AtPRMT5 in Prp19C/NTC complex recruitment to the spliceosome during pre-mRNA splicing.