Fig. S1.

mPFC sends direct monosynaptic inputs to the DRN. (A, 1) Schematic of Fluoro-Gold injection in the DRN and subsequent retrograde transport of Fluoro-Gold through the mPFC axon terminal. (A, 2) Confocal image of a coronal brain slice containing the mPFC. (Inset) Two-photon image of the Fluoro-Gold–expressing cells bodies in the mPFC. Note the dense labeling with Fluoro-Gold of deep-layer cells in the mPFC. (A, 3) Confocal image of Fluoro-Gold in the DRN 1 wk postinjection. Aq, aqueduct.