Fig. S3.

Differential overall gene-expression level analyses in full-length PSI male fly heads and PSIΔAB male fly heads. (A) Flowchart of differential gene-expression level analyses in full-length PSI and PSIΔAB mutant fly heads using DESeq2. (B) GO analysis of the differentially expressed genes in PSIΔAB male fly head compared with full-length PSI. Categories of related genes are listed at the left and enrichment significance (−log FDR) is indicated along the x axis. (C) The FPKM values (normalized overall gene-expression level, fragments per kilobase per million) of all of the differentially alternatively spliced male courtship regulatory genes. Almost all of the gene transcripts maintain the same overall expression level in full-length PSI and PSIΔAB male fly heads.