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. 2016 Apr 25;113(19):5424–5428. doi: 10.1073/pnas.1604885113

Fig. 4.

Fig. 4.

Expression of Hsp110 protein in motor neurons of Thy1-Hsp110/G85R SOD1YFP strain determined by immunostaining. Twenty-micrometer transverse lumbar sections of fixed spinal cords were prepared from 3-mo-old mice of the G85R SOD1YFP strain or of Thy1-Hsp110/G85R SOD1YFP strain and immunostained with a polyclonal antibody directed against a peptide from the C-terminal region of human Hsp110 (Santa Cruz Biotechnology), as described in SI Methods. (Left) YFP-fluorescent large motor neurons observed in the ventral horn. (Right) Fluorescence of the motor neurons in the red channel from anti-Hsp110 immunostaining, showing weak signals from endogenous mouse Hsp110 in sections from the G85R SOD1YFP strain and strong signals in sections from the Thy1-Hsp110/G85R SOD1YFP strain. This observation is consistent with the presence of additional Hsp110 protein in the motor neurons of the double transgenic mice. See text for details. (Scale bar: 50 µm.)