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. 2016 Apr 25;113(19):5424–5428. doi: 10.1073/pnas.1604885113

Fig. S2.

Fig. S2.

Survival curves of G85R SOD1YFP and bichaperone-containing G85R SOD1YFP strain containing transgenic Thy1-Hsp110 and Thy1-Hsc70 (see SI Methods for derivation). The extension of survival of the bichaperone-containing G85R SOD1YFP mice (red trace) is similar to that of the Thy1-Hsp110/G85R SOD1YFP mice (Fig. 1). Note that with producing founder lines of Thy1-Hsc70, we could not obtain copy numbers above ∼20, thus allowing a copy number of only ∼40 with homozygosis. At the RNA level, analysis of two independent lines, both hemizygous and homozygous, showed an increase of only 50–75% over endogenous mouse Hsc70, and an increase of protein could not be observed by immunostaining or Western blot analysis. Thus, there appears to be a level of regulation that prevents overproduction of Hsc70 and may explain inability here to prolong survival. Alternatively, Hsc70 may be amply present and not comprise a rate-limiting step in the disaggregation cycle.