Table S4.
pBIG2 constructs in this study
| pBIG2 construct | Alias | Vector | PGC1 | PGC2 | PGC3 | PGC4 | PGC5 | Construct size, kb | Shown in figure | Clones correct/analyzed |
| FW_II-1 | APC/C–Emi1 | pBIG2abcde | FW_I-1 | FW_I-2 | FW_I-3 | FW_I-4 | FW_I-5 | 43.6 | Fig. 2A and Fig. S5C | 6/6 |
| FW_II-2 | APC/C | pBIG2abcd | FW_I-1 | FW_I-2 | FW_I-3 | FW_I-4 | — | 38.3 | Fig. 2B | 1/4 |
| FW_II-3 | Platform | pBIG2ab | FW_I-1 | FW_I-2 | — | — | — | 23.7 | Fig. 2B | 2/4 |
| FW_II-4 | Platform+8 | pBIG2ab | FW_I-1 | FW_I-6 | — | — | — | 26.0 | Fig. 2B | 2/4 |
| FW_II-5 | Arclamp | pBIG2abcd | pBIG1a(empty) | pBIG1b(empty) | FW_I-3 | FW_I-4 | — | 21.0 | Fig. 2B | 1/1 |
| FW_II-6 | Arclamp(6-Strep) | pBIG2abcd | pBIG1a(empty) | pBIG1b(empty) | FW_I-7 | FW_I-4 | — | 21.1 | Fig. 2B | 1/1 |
| FW_II-7 | APC/C(3m) | pBIG2abcd | FW_I-1 | FW_I-2 | FW_I-3 | FW_I-8 | — | 38.3 | Fig. 2B | 2/4 |
| FW_II-8 | APC/C(8m) | pBIG2abcd | FW_I-1 | FW_I-2 | FW_I-3 | FW_I-9 | — | 38.3 | Fig. 2B | 1/1 |
| FW_II-9 | APC/C(10m) | pBIG2abcd | FW_I-1 | FW_I-2 | FW_I-3 | FW_I-10 | — | 38.3 | Fig. 2B | 1/1 |
| FW_II-10 | APC/C(3/8m) | pBIG2abcd | FW_I-1 | FW_I-2 | FW_I-3 | FW_I-11 | — | 38.3 | Fig. 2B | 5/10 |
| FW_II-11 | APC/C(3/10m) | pBIG2abcd | FW_I-1 | FW_I-2 | FW_I-3 | FW_I-12 | — | 38.3 | Fig. 2B | 1/1 |
| FW_II-12 | APC/C(8/10m) | pBIG2abcd | FW_I-1 | FW_I-2 | FW_I-3 | FW_I-13 | — | 38.3 | Fig. 2B | 1/4 |
| FW_II-13 | APC/C(3/8/10m) | pBIG2abcd | FW_I-1 | FW_I-2 | FW_I-3 | FW_I-14 | — | 38.3 | Fig. 2B | 1/1 |
| FW_II-14 | APC/C(Δ10) | pBIG2abcd | FW_I-1 | FW_I-2 | FW_I-3 | FW_I-15 | — | 37.1 | Fig. 2B | 1/1 |
| FW_II-15 | Kinetochore MN | pBIG2ab | FW_I-19 | FW_I-21 | — | — | — | 19.5 | Fig. 3K and Fig. S5B | 6/6 |
| FW_II-16 | Kinetochore KMN | pBIG2ab | FW_I-22 | FW_I-23 | — | — | — | 24.5 | Fig. 3K and Fig. S5B | 6/6 |