Fig. S1.

RT-PCR analysis of the expression of the atypical gene cluster of Mmc GM12. To assess whether the eight genes of the cluster were expressed, a RT-PCR experiment was performed. The eight primer pairs designed to specifically amplify a fragment of each of the genes were first validate using genomic DNA (gDNA) as a PCR template. The presence of cDNA reverse transcribed from mRNA was then assessed (cDNA). As a control, an identical PCR was performed using non–retrotranscribed RNA (−RT) to validate the lack of DNA contamination in the RNA samples.