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. 2016 Apr 13;113(19):5364–5369. doi: 10.1073/pnas.1602886113

Fig. 3.

Fig. 3.

Enhancer L is necessary for HLA-G expression in primary extravillous trophoblasts (EVTs). (A) Transduction of first-trimester HLA-G+ EVTs with lentiviral Cas9 and Enhancer L gRNAs, assessed based on GFP expression. BF, bright-field (40× magnification). (B) Reduction of HLA-G expression at the mRNA level following Enhancer L deletion. Bars represent average ± SEM of three independent experiments. Gene expression normalized to GAPDH expression. *P < 0.05, paired Student’s t test. EL, Enhancer L. (C) Enhancer L deletion leads to significant reduction in HLA-G surface expression in primary EVTs, as assessed by FACS. One representative experiment is shown (n = 3). (D) Significant reduction in HLA-G surface expression upon Enhancer L deletion in primary EVTs [mean fluorescence intensity (MFI)]. Bars represent average ± SEM of three independent experiments. *P < 0.05, paired Student’s t test.