Fig. S4.
Kinetic analysis showing that phospho-mimetics enhance the activity of APC/CCDC20. (A) Curve fits and kinetic parameters from titrating CDH1 to test the role of APC/C mutants pA and pE on catalytic efficiency of Ub-securin ubiquitination with UBE2S and UBE2C. SEM, n ≥ 3. (B) Bar graphs showing the effect of pA and pE on the concentrations of CDC20 and CDH1 required for half-maximal activation and normalized activity in Ub-securin ubiquitination assays with UBE2S and UBE2C, respectively.