Fig. S4.

Pharmacodynamic effects of chimeric activator variants on reticulocytes and reticulated platelets in huGYPA transgenic mice. This experiment is another repeat of that in Fig. 3 but with six mice per dose group. (A) huGYPA transgenic mice received a single i.p. injection of darbepoetin, 10F7-EPO variant, or saline vehicle at the indicated concentrations (1 pmol darbepoetin = 37 ng; 1 pmol 10F7-EPO variant = 72 ng). Blood samples were removed on days 0, 4, and 7 postdosing. Hematologic parameters measured by flow cytometry were reticulocyte fraction (B–E) and reticulated platelet fraction (F–I). Measurements were background-subtracted relative to day 0. Graphs display mean ± SEM (n = 6).