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. Author manuscript; available in PMC: 2016 May 16.
Published in final edited form as: Leuk Lymphoma. 2014 Aug 4;56(2):450–459. doi: 10.3109/10428194.2014.922180

Fig 2. CLL patient cells are sensitized to fatty acid oxidation inhibitor upon glucose deprivation. CLL is not impacted by glutamine deprivation or inhibition of autophagy.

Fig 2

All culture and treatment was for 48 hours. Cell viability assessed by AnnexinV and DAPI staining A - CLL patient cells (n=6), cultured in a) 5mM glucose media, b) glucose-free media and c) glutamine-free media+ 5mM glucose. B - CLL patient cells (n=4) cultured in glucose-containing and glucose-free media in the presence and absence 10µm chloroquine. C - CLL patient cells (n=3), cultured in glucose-containing and glucose-free media in the presence and absence for 10µm etomoxir. D – CLL patient cells (n=4) cultured in glucose containing or glucose-free media in the presence and absence of 10mM galactose or 2 methylpyruvate. All samples were normalized to viability of CLL patient cells cultured in 5mM glucose-containing media. E: CLL patient cells (n=7), cultured in glucose containing or glucose-free media, supplemented with 10mM galactose and treated with 5 mM metformin. *p <0.05 *** p <0.005****p <0.0001.