Figure 6. Persistent clonal lineages of Env- and CD4bs-specific repertoires from animals F125 & F128 have established during the immunization.

Bar graph, the frequency of expansions from each individual clonal lineage at different immunization time points. Clonal lineages isolated at Imm 2 are in blue and Imm 5 in purple. The clonal lineages, which have expansions at both time points, are highlighted with yellow boxes. Dot lines depict the frequency of clonal lineages with single expansion in the repertoire at each time point. Pie chart, the persistence of clonal lineages of the Env- and CD4bs-specific repertories. Clonal lineages that have expansions at both time points are referred as highly persistent and designated as Pst^hi compartment (yellow), that have expansions only at Imm 2 were classified as of low persistence (Pst^lo, blue), while lineages have expansions only at Imm 5 were designated as Newly emerging (purple) compartment. The frequencies of these three categories of clonal lineages with different clonal persistence out of the total clonal lineages were indicated. Env-specific lineages are shown in the upper two panels and CD4bs lineages in the lower two panels.