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. Author manuscript; available in PMC: 2017 May 16.
Published in final edited form as: Integr Biol (Camb). 2016 Mar 21;8(5):654–664. doi: 10.1039/c5ib00284b

Fig. 3. Validation of single tumor cell detection and collection in 96-well plate.

Fig. 3

A) Passage time vs. buoyant mass measurements of H1650 cells spiked into enriched mononuclear blood cells. The cells included in the boxed region (> 50pg buoyant mass & > 0.01 s passage time) were imaged on-chip (B) as well as collected off-chip for additional verification (C). Gray dots outside of the boxed region represent blood cells that were also measured in the SMR during the experiment. B) Images taken on-chip, in the exit channel of the SMR, immediately after custom software detected a long passage time signal. It was confirmed that all detected SMR signals falling into the boxed region of interest (A) corresponded to an H1650 tumor cell by immunofluorescence imaging (EpCAM/CDH11+ and CD45−). C) Each detected H1650 cell was successfully collected off-chip in a 96-well plate. Based on additional immunofluorescence imaging of each well, a tumor cell was identified in each well (EpCAM/CDH11+ and CD45−), along with a few leukocytes (CD45+ and EpCAM/CDH11−).