Table 2. Collected measurement events from cell line-spiked blood samples.
For cells to be considered positive for CD45 or EpCAM, they had to have a clear ring of fluorescence, indicating surface expression of the protein. If no clear ring of fluorescence was present at the boundary of the cell, general fluorescence was considered to be background, nonspecific fluorescence. WBC = white blood cell.
| Spiked Cells |
Well Label of Collected Peak |
# Cells | Well Content Description |
|---|---|---|---|
| LNCaP | L1 | 2 | 1 DAPI−CD45−EpCAM+ (LNCaP) 1 DAPI+CD45+EpCAM− (Unidentified WBC) |
| L2 | 1 | DAPI+CD45−EpCAM+ (LNCaP) | |
| L3 | 1 | DAPI+CD45−EpCAM+ (LNCaP) | |
| L4 | 1 | DAPI+CD45−EpCAM+ (LNCaP) | |
| L5 | 0 | Debris | |
| L6 | 0 | Debris (Not in region of interest) | |
| L7 | 1 | DAPI+CD45−EpCAM− (Activated neutrophil, not in region of interest) |
|
| PC3 | P1 | 1 | DAPI+CD45−EpCAM+ (PC3) |
| P2 | 2 | 2 DAPI+CD45−EpCAM+ (PC3) | |
| P3 | 1 | DAPI+CD45−EpCAM+ (PC3) | |
| P4 | 0 | Debris | |
| P5 | 1 | DAPI+CD45−EpCAM+ (PC3) | |
| P6 | 1 | DAPI+CD45−EpCAM+ (PC3) | |
| P7 | 1 | DAPI+CD45−EpCAM− (Unidentified cell) | |
| P8 | 0 | Debris | |
| P9 | 2 | 2 DAPI+CD45−EpCAM+ (PC3) | |