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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: J Immunol. 2016 Mar 14;196(9):3828–3833. doi: 10.4049/jimmunol.1500532

Figure 3. Neutrophil chemotaxis.

Figure 3

A. Neutrophils were injected into the gradient maker coated with ICAM-1. B. and tracked (black lines) chemotaxis of the neutrophils was observed by CCD camera. C. The migration paths of 80–110 wild type, Gαi2 deficient or Gαi3 deficient neutrophils in the gradient maker. Scale bar = 40 μm. D. The total path length migrated by wild type, Gαi2 deficient or Gαi3 deficient neutrophils in the gradient maker during 40 min. E. The chemotactic index (upgradient/total path length) of wild type, Gαi2 deficient or Gαi3 deficient neutrophils. Data are presented as mean ± SEM. ** or * indicates P<0.01 or P<0.05 from wild type neutrophils respectively. Data are representative of at least three independent experiments.