Skip to main content
. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: J Immunol. 2016 Mar 14;196(9):3828–3833. doi: 10.4049/jimmunol.1500532

Figure 5. Gαi3 regulates PI3K activation after CXCL1 stimulation in neutrophils.

Figure 5

A. Gαi2 or Gαi3 deficient neutrophils were stimulated with the indicated concentrations of CXCL1 and lysates were immunoblotted with an antibody to phospho-AKT (p-AKT). The membranes were stripped and immunoblotted with pan-AKT as a loading control. B. The values in the bar graph represent mean ± SEM relative intensities as determined by densitometry (wild type neutrophils with 10 ng/ml CXCL1 stimulation=1). * indicates P<0.05 from wild type neutrophils. Data are representative of at least three independent experiments.