Spore germination and yeast growth are affected differently by antifungals and other chemical compounds. SD containing amphotericin B (1 μg/mL), fluconazole (16 μg/mL), benomyl (62.5 μg/mL), or bortezomib (960 μg/mL) was applied to yeast (A, D) and spores (B, E). Yeast growth was measured by normalized frame coverage (A, D) while germination was measured both with population composition (B, E) and 2D histograms of cell area and aspect ratio (C, F). 2D histograms include cell populations from three independent wells. * indicates p-value <0.05 compared to SD, and error bars represent standard deviation.