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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Kidney Int. 2016 Apr 14;89(6):1281–1292. doi: 10.1016/j.kint.2016.01.030

Fig 4. Increased interstitial fibrosis after overexpression of EST and TG2 by subcapsular injection into kidney.

Fig 4

(A) Fourteen days after kidney lower pole injection either with buffer, 2ug of EST peptide, 1 ug of TG2 or a combination of TG2 and EST dissolved in 10 μl buffer. TG2 together with EST injected mouse kidneys show increased interstitial fibrosis as analyzed by picrosirius red positive area staining. Representative images for each group are shown at magnification of ×400. Right panel: Quantification of interstitial fibrosis area was performed using integrated density by Image J at ×100 magnification. Data are mean ± SEM. *P<0.05, n=3~4 animals per group. (B) Interstitial fibrosis in kidneys of transgenic mice overexpressing EST. Collagen expression is increased in the EST overexpressing mouse kidneys already at the age of 2–3 months and it is further enhanced in 10–12 months-old mice. Left panel: Representative images for each group are shown at magnification of ×100. Right panel: Quantification of integrated density using NIH image J software. Magnification ×100. Data are means ± SEM. **P<0.01, *P<0.05. n= 5 animals per group.