Figure 7. Effects of BRD7-dependent XAF1 expression in vivo .
A. A549 cells were transfected by BRD7 siRNA (siBRD7) or scramble siRNA and then each of the cells was treated with IFN-γ (100ng/ml). Expression of XAF1 and BRD7 proteins were confirmed by Western blotting. B. Each of the cells was irradiated with 0, 1, 2 and 4 Gy of IR, and incubated about 2 weeks for colony formation. Cell survival values were normalized to those of the unirradiated cells. *p < 0.05 versus BRD7-depleted group. C. Effect of IFN-γ administration on growth of A549 tumor xenografts in nude mice. Nude mice (n = 10 per group) were implanted subcutaneously with A549 and groups of mice received vehicle or IFN-γ (1 × 105 unit) three times for a week. Tumor size was assessed by direct caliper measurement. Data are means ± SE, n = 10. Tumor volume between shCon and shBRD7 in IFN-γ treated group was statistically significantly different at *p < 0.05 by two-sided Student's t-test. D. Xenograft tumors were embedded with paraffin, were serially sectioned and stained with hematoxylin and eosin (H&E). The tissue samples were immunostained with XAF1 and BRD7 antibodies and observed with fluorescence microscope.