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. 2015 Dec 11;7(5):5273–5288. doi: 10.18632/oncotarget.6567

Figure 3. PMP22-ELOVL5 gene fusion.

Figure 3

a. Schematic overview of the location of the genomic breakpoint and the resulting fusion transcript identified in the cell line IOR/OS15. Validation of the fusion transcript by Sanger sequencing confirmed the breakpoint as illustrated in the sequence chromatogram. b. Double-fusion FISH picture of IOR/OS15 in tissue microarray (cell pellets). Green and red probes identify PMP22 and ELOVL5, respectively. Colocalized signals are visible in yellow and confirm the gene fusion at the genome level. Further, normalized expression patterns of PMP22 c. and ELOVL5 d. are shown for IOR/OS15, differentiated and undifferentiated iMSC#3 with the corresponding FPKM (Fragments Per Kilobase of exon per Million fragments mapped) calculated using Cufflinks. For IOR/OS15 a reduced expression of PMP22 exon 5 and ELOVL5 exon 1 is visible, caused by the loss of these exons in the fusion transcript.