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. 2015 Dec 30;7(5):5598–5612. doi: 10.18632/oncotarget.6798

Figure 4. ENO1 silencing impairs cancer cell growth.

Figure 4

A. Cell proliferation analysis of CFPAC-1 (left panel), MDA-MB-231 (middle panel) and NCI-H441 (right panel) cell lines growth after transduction with shCTRL (white circles) or shENO1 (black circles). Cells were starved and counted every 24 h after serum replenishment. Curves were compared by two-way ANOVA, ***p < 0.001. B. Cell survival assessed by MTT assay. Cells transduced with shCTRL (white bars) or shENO1 (black bars) were starved and MTT solution was added 48 h after serum replenishment. OD values were measured at 570 nm. C. Flow cytometry cell cycle analysis of serum-starved shCTRL and shENO1 CFPAC-1 cells at the indicated time points after serum replenishment. Graphs represent the percentage of cells at each phase. D. Western blot analysis of shCTRL and shENO1 CFPAC-1 cells with the indicated antibodies. Cells were serum starved and lysed at 24 h after serum replenishment. β-actin was used as a loading control. One representative out of three independent experiments is shown. Results are reported in the graphs as means of three independent experiments ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 relative to shCTRL.