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. 2015 Dec 29;7(5):5690–5701. doi: 10.18632/oncotarget.6788

Figure 3. TSHZ2 is a CtBP-dependent transcriptional repressor.

Figure 3

A. Immunoprecipitation analysis. HEK293T cells were transiently transfected with the indicated expression vectors and used in immunoprecipitation analysis. IgH indicates the immunoglobulin heavy chain of the anti-myc tag antibody weakly cross-reacted to a secondary antibody for immunodetection. B. In situ PLA was performed using tissue sections cut from human mammary tissue blocks. DAPI was used to mark nuclei. Bar, 10 μm. C. Luciferase reporter assay. Cells were transiently transfected with either GAL4 or GAL4-fused TSHZ2 expression vector (wild-type or mutCIM) along with pG5TKluc, UAS-containing thymidine kinase minimal promoter-driven luciferase vector, and Renilla luciferase vector. Columns, means of three independent experiments; bars, SD. *P < 0.01.