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. 2015 Dec 29;7(5):5690–5701. doi: 10.18632/oncotarget.6788

Figure 5. TSHZ2 suppresses the transcriptional activity of GLI1.

Figure 5

A. Immunoprecipitation analysis. HEK293T cells were transiently transfected with the indicated expression vectors and used in immunoprecipitation analysis. B. In situ PLA was performed using tissue sections cut from human mammary tissue blocks. DAPI was used to mark nuclei. Bar, 10 μm. C. Luciferase reporter assay. Cells were transiently transfected with the indicated expression vectors and either wtGBSx4TKLuc or mtGBSx4TKLuc. To normalize the transfection efficiency, Renilla luciferase vector was also transfected. Columns, mean of three independent experiments; bars, SD. *P < 0.01. D. Immunoblot analysis of siRNA-transfected HMEC4htertshp16 cells.